Kedu ihe kpatara enwere ike ijikwa tubes serum iji chọpụta ọdịnaya D-dimer?A ga-enwe mkpụkọ fibrin n'ime tube serum, ọ bụ na ọ gaghị eweda ya ka ọ bụrụ D-dimer?Ọ bụrụ na ọ naghị eweda ya ala, gịnị kpatara enwere mmụba dị ukwuu na D-dimer mgbe a na-emepụta mkpụkọ ọbara na tube anticoagulation n'ihi nlele ọbara dara ogbenye maka nyocha coagulation?
Nke mbụ, nchịkọta ọbara na-adịghị mma nwere ike iduga mmebi endothelial vaskụla, na ntọhapụ nke ihe na-eme ka anụ ahụ subendothelial na ụdị plasminogen activator (tPA) dị n'ime ọbara.N'otu aka ahụ, ihe na-akpata anụ ahụ na-eme ka ụzọ coagulation exogenous na-emepụta mkpirisi fibrin.Usoro a dị ngwa ngwa.Naanị lelee oge prothrombin (PT) ka ị mara, nke na-adịkarị ihe dịka 10 sekọnd.N'aka nke ọzọ, mgbe emechara fibrin, ọ na-arụ ọrụ dị ka onye na-emekọ ihe iji mụbaa ọrụ nke tPA site na 100 ugboro, na mgbe tPA jikọtara n'elu fibrin, ọ gaghị adị mfe igbochi plasminogen activation inhibitor-1. PAI-1).Ya mere, plasminogen nwere ike gbanwee ngwa ngwa ma na-aga n'ihu na-agbanwe ghọọ plasmin, mgbe ahụ, fibrin nwere ike imebi ya, a pụkwara ịmepụta nnukwu FDP na D-Dimer.Nke a bụ ihe kpatara na mmepụta nke mkpụkọ ọbara na vitro na ngwaahịa mmebi fibrin na-abawanye nke ukwuu n'ihi nlele ọbara dara ogbenye.
Mgbe ahụ, gịnị kpatara nchịkọta nkịtị nke serum tube (na-enweghị ihe mgbakwunye ma ọ bụ nke nwere coagulant) ụdịdị na-emepụtakwa fibrin clots na vitro, ma ọ dịghị eweda ya iwepụta nnukwu FDP na D-dimer?Nke a dabere na tube serum.Ihe mere mgbe a chịkọtachara ihe atụ: Nke mbụ, ọ dịghị nnukwu ego nke tPA na-abanye n'ọbara;nke abụọ, ọ bụrụgodị na obere tPA na-abanye n'ọbara, a ga-ejikọta tPA n'efu site na PAI-1 wee hapụ ọrụ ya n'ime ihe dị ka nkeji 5 tupu ya ejikọta ya na fibrin.N'oge a, a naghị emepụta fibrin n'ime tube serum na-enweghị ihe mgbakwunye ma ọ bụ na-eji coagulant.Ọ na-ewe ihe karịrị nkeji iri maka ọbara na-enweghị ihe mgbakwunye iji chịkọta ọnụ, ebe ọbara nwere coagulant (na-abụkarị silicon ntụ ntụ) na-amalite n'ime.Ọ na-ewekwa ihe karịrị nkeji ise iji mepụta fibrin site n'okporo ụzọ coagulation ọbara.Na mgbakwunye, ọrụ fibrinolytic na ụlọ okpomọkụ na vitro ga-emetụtakwa.
Ka anyị kwuo banyere thromboelastogram ọzọ n'isiokwu a: ị nwere ike ịghọta na ọbara ọgbụgba na tube serum adịghị mfe iweda ya, ma ị nwere ike ịghọta ihe mere ule thromboelastogram (TEG) adịghị emetụ n'ahụ iji gosipụta hyperfibrinolysis - ọnọdụ abụọ ahụ yiri, N'ezie, okpomọkụ n'oge ule TEG nwere ike ịnọgide na-enwe na 37 degrees.Ọ bụrụ na TEG na-enwe mmetụta karị iji gosipụta ọnọdụ fibrinolysis, otu ụzọ bụ ịgbakwunye tPA na in vitro TEG nnwale, ma a ka nwere nsogbu nhazi na ọ dịghị ngwa zuru ụwa ọnụ;na mgbakwunye, enwere ike tụọ ya n'akụkụ akwa ozugbo emechara nlele, mana nsonaazụ ya dịkwa oke oke.Nnwale ọdịnala na nke dị irè maka ịlele ọrụ fibrinolytic bụ oge mgbasa nke euglobulin.Ihe kpatara uche ya dị elu karịa nke TEG.N'ime ule ahụ, a na-ewepụ ihe mgbochi plasmin site n'ịgbanwe nha pH na centrifugation, mana ule na-eri Ọ na-ewe ogologo oge ma dịtụ ike, ọ na-adịkarịghị eme ya na ụlọ nyocha.
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