Ihe nyocha ọbara Rheology akpaghị aka zuru oke

Okwu mmalite nke nyocha

SA-6900 akpaaka ọbara rheology analyzer nakweere ụdị cone/plate ụdị nha mode.Ngwaahịa a na-amanye nrụgide a na-achịkwa na mmiri nke a ga-atụle site na moto torque dị ala.A na-edobe ogwe draịva ahụ n'ebe etiti site na nguzogide magnetik levitation dị ala, nke na-ebufe nrụgide etinyere na mmiri nke a ga-atụle na isi ihe atụ ya bụ ụdị cone-plate.Kọmputa na-achịkwa nri nri niile na-akpaghị aka.Enwere ike ịtọ ọnụ ọgụgụ shear na-enweghị usoro n'ogo nke (1～200) s-1, ma nwee ike ịchọpụta akụkụ akụkụ abụọ maka ọnụọgụ shiee na viscosity ozugbo.A na-esetịpụ ụkpụrụ nha na Newton Viscidity Theorem.

Nkọwa nka nka

Akpachara anya maka nchịkọta sample na nkwadebe

1. Nhọrọ na usoro onunu ogwu nke anticoagulant

1.1 Nhọrọ nke anticoagulant: Ọ bụ ihe amamihe dị na ya ịhọrọ heparin dị ka ọgwụ mgbochi.Oxalate ma ọ bụ sodium citrate nwere ike ime ka mma cell shrinkage na-emetụta mkpokọta na nrụrụ nke mkpụrụ ndụ ọbara uhie, na-akpata mmụba ọbara viscosity, ya mere ọ dịghị mma maka ojiji.

1.1.2 dose nke anticoagulant: heparin anticoagulant itinye uche bụ 10-20IU / mL ọbara, siri ike na-adọ ma ọ bụ elu itinye uche mmiri mmiri na-eji maka anticoagulation Agent.Ọ bụrụ na ejiri ọgwụ anticoagulant mee ihe ozugbo, ekwesịrị ịtụle mmetụta dilution ya na ọbara.Otu ogbe nke ule kwesịrị

Jiri otu anticoagulant nwere otu nọmba ogbe.

1.3 mmepụta nke tube anticoagulant: ọ bụrụ na a na-eji ọgwụ mgbochi mmiri na-eme ihe, a ga-etinye ya na tube iko ma ọ bụ karama iko ma kpoo ya na oven Mgbe ihichachara, a ghaghị ịchịkwa okpomọkụ nke ihicha na ihe karịrị 56 ° C.

Rịba ama: Ọnụ ọgụgụ nke anticoagulant ekwesịghị ịdị ukwuu iji belata mmetụta dilution na ọbara;Ọnụ ọgụgụ nke anticoagulant ekwesịghị ịdị ntakịrị, ma ọ bụghị ya, ọ gaghị erute mmetụta anticoagulant.

2. Nchịkọta ihe atụ

2.1 Oge: N'ozuzu, a ga-anakọta ọbara n'isi ụtụtụ na afọ efu na n'ọnọdụ dị jụụ.

2.2 Ebe: Mgbe ị na-ewere ọbara, nọrọ ọdụ ma were ọbara si n'ikpere aka ihu venous.

2.3 Mbelata oge mgbochi venous dị ka o kwere mee n'oge nchịkọta ọbara.Mgbe agịga basịrị n'ime arịa ọbara, tọpụ ngwa ngwa ahụ cuff ka ọ nọrọ jụụ Ihe dị ka sekọnd ise ka ịmalite nchịkọta ọbara.

2.4 Usoro nchịkọta ọbara ekwesịghị ịdị ngwa ngwa, yana enwere ike izere mmebi nke mkpụrụ ndụ ọbara uhie nke ike ịkpachasị ahụ kpatara.Maka nke a, lancet dị n'ime dayameta nke ọnụ ọnụ ka mma (ọ ka mma iji agịga n'elu 7 nlele).Ọ bụghị ihe amamihe dị na ya ịdọrọ ike dị ukwuu n'oge nchịkọta ọbara, iji zere ike ịkpacha ihe na-adịghị mma mgbe ọbara na-agafe na agịga.

2.2.5 Ngwakọta ihe atụ: Mgbe a na-achịkọta ọbara ahụ, wepụ agịga injection ahụ, were nwayọọ nwayọọ tinye ọbara n'ime tube ule n'akụkụ mgbidi nke tube ule ahụ, wee jide n'etiti tube ule ahụ n'aka wee tee ya ma ọ bụ slide ya na mmegharị okirikiri na tebụl iji mee ka ọbara gwakọta ya na ọgwụ mgbochi.

Iji zere mkpụkọ ọbara, ma zere ịma jijiji siri ike iji zere hemolysis.

3.Nkwadebe nke plasma

Nkwadebe plasma na-anabata usoro ọgwụgwọ oge niile, ike centrifugal dị ihe dị ka 2300 × g maka nkeji 30, a na-ewepụta akwa akwa ọbara nke Pulp, maka ịlele viscosity plasma.

4. Ntụnye ihe atụ

4.1 Okpomọkụ nchekwa: enweghị ike ịchekwa ụdị n'okpuru 0 Celsius.N'okpuru ọnọdụ oyi, ọ ga-emetụta ọnọdụ physiological nke ọbara.

State na rheological Njirimara.Ya mere, a na-echekwa ihe nlele ọbara na ụlọ okpomọkụ (15°C-25°C).

4.2 Oge ntinye: A na-enyocha ihe nlele ahụ n'ime awa 4 n'ime ụlọ okpomọkụ, ma ọ bụrụ na a na-ewere ọbara ozugbo, ya bụ, ọ bụrụ na a na-eme ule ahụ, nsonaazụ nke ule ahụ dị ala.Ya mere, ọ dị mma ikwe ka ule ahụ guzoro maka nkeji 20 mgbe ị nwesịrị ọbara.

4.3 Agaghị ajụ oyi ma chekwaa n'okpuru 0 Celsius.Mgbe a ga-echekwa ihe nlele ọbara ruo ogologo oge n'okpuru ọnọdụ pụrụ iche, a ga-akara ha akara Tinye ya na friji na 4 ℃, na oge nchekwa n'ozuzu adịghị ihe karịrị awa 12.Chekwaa ihe nlere anya nke ọma tupu anwale, maa jijiji nke ọma, na ọnọdụ nchekwa kwesịrị igosi na akụkọ nsonaazụ.